Nucleic Acids Research, 1983, Vol. 11, No. 12 4049-4063
© 1983
MOLECULAR BIOLOGY |
Saccharomyces cerevisiae contains two discrete genes coding for the 
-factor pheromone
Department of Molecular Biology, Genentech, Inc. 460 Point San Bruno Blvd., South San Francisco, CA 94080, USA
Received March 21, 1983. Revised May 13, 1983. Accepted May 13, 1983.
Two genes, MF
1 and MF2, coding for the -factor in yeast Saccharomyces cerevisiae were identifTed by in situ colony hybridization of synthetic probes to a yeast genomic library. The probes were designed on the basis of the known amino acid sequence of the tridecapeptide -pheromone. The nucleotide sequence revealed that the two genes, though similar in their overall structure, differ from each other in several striking ways. MFl gene contains 4 copies of the coding sequence for the -factor, which are separated by 24 nucleotides encoding the octapeptide Lys-Arg-Glu-Ala-Glu(or Asp)-Ala-Glu-Ala. Tne first -factor coding block is preceded by a sequence for the hexapeptide Lys-Arg-Glu-Ala-Glu-Ala and 83 additional amino acids. MF2 gene contains coding sequences for two copies of the -factor that differ from each other and from -factor encoded by MF1 gene by a Gln 
Asn and a Lys Arg substitution. Tne first copy of the -factor is preceded by a sequence coding for 87 amino acids which ends with Lys-Arg-Glu-Ala-Val-Ala-Asp-Ala. The coding blocks of the two copies of the pheromone are separated by the sequence for Lys-Arg-Glu-Ala-Asn-Ala-Asp-Ala. Thus, the -factor can be derived from 2 different precursor proteins of 165 and 120 amino acids containing, respectively, 4 and 2 copies of the pheromone.
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