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Nucleic Acids Research, 1983, Vol. 11, No. 12 4157-4175
© 1983

MOLECULAR BIOLOGY

Regions specifying transcriptional termination and pausing in the bacteriophage SP01 terminal repeat

Sean M. Brennan* and E.Peter Geiduschek

Department of Biology, B-022 University of California San Diego, La Jolla, CA 92093, USA

Received January 18, 1983. Revised May 13, 1983. Accepted May 13, 1983.

We have determined the nucleotide sequences of four termination sites recognized by Bacillus subtilis RNA polymerase. These sites are located in the terminally repeated segment of the bacteriophage SP01 genome, where most early phage transcription occurs. The SPO1 terminators have structures that are similar to those recognized by Escherichia coli RNA polymerase, containing a region of dyad symmetry followed by a stretch of HMD residues in the noncoding DNA strand (HMU is substituted for T in SP01 DNA). We note that in a terminator that is only 60% efficient in vitro, there is a greater distance between these two conserved elements than exists in more efficient terminators.

We also find that RNA polymerase molecules which elongate a transcript through a partial terminator often pause at this site.

*Present Address: MRC Laboratory of Molecular Biology Hills Road Cambridge, CB2 2QH England


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