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Nucleic Acids Research, 1983, Vol. 11, No. 13 4541-4552
© 1983

MOLECULAR BIOLOGY

Carp preproinsulin cDNA sequence and evolution of insulin genes

V. Hahn, J. Winkler+, T.A. Rapoport, D.-H. Liebscher, Ch. Coutelle and S. Rosenthal

+Mathematical Biology, Central Institute of Molecular Biology, Academy of Sciences of the GDR 1115 Berlin-Buch, GDR Departments of Cell Differentiation and Academy of Sciences of the GDR 1115 Berlin-Buch, GDR

Received April 15, 1983. Accepted June 17, 1983.

The nucleic acid sequence of the preproinsulln cDNA of carp (Cyprinus carpio), cloned in the PstI-site of pBR322 (i), has been determined. The sequenced insert of 439 bp includes the complete coding Information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. The nucleotlde sequence confirms the previously established amino acid sequence of carp insulin (2) and determines those of the signal (21 aa 1) and C-peptide (35 aa 1). The observed shortness of the signal peptide of carp preproinsulin and the N-terminal addition of 2 amino acids to the carp insulin B-chain suggest that the cleavage site of the signal peptidase has moved.

Calculations based on the comparison of known preproinsulin cDNA sequences showed that the evolutionary distance between fresh water and salt water teleostians is not smaller than between man and chicken.


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