Skip Navigation

This Article
Right arrow Print PDF (367K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Rio, P.
Right arrow Articles by Leng, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rio, P.
Right arrow Articles by Leng, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 14 4947-4956
© 1983

MOLECULAR BIOLOGY

Preferential binding of the chemical carcinogen N-hydroxy-2-aminofluorcne to B-DNA as compared to Z-DNA

Pascale Rio and Marc Leng

Centre de Biophysique Moléculaire, CNRS, 1A, avenue de la Recherche Scientifique 45045 Orleans Cedex, France

Received May 17, 1983. Revised June 23, 1983. Accepted June 23, 1983.

The reaction between the chemical carcinogen N-hydroxy-2-aminofluorene and poly (dG-dC).poly(dG-dC)(B-form), poly(dG-m5dC).poly(dG-m5dC)(B-or Z-form), poly(dG-br5dC).poly(dG-br5dC) (Z-form) has been studied. The carcinogen binds covalently to B-DNA but does not bind significantly to Z-DNA. These results arc discussed as related to the accessibility, the electrostatic potential and the dynamic structure of DNA. The accessibility and the electrostatic potential of DNA do not explain the difference in reactivity of the carcinogen since a related carcinogen N-acetoxy-N-acety1-2-aminofluorene binds equally well to both B and Z-DNA. On the other hand, poly(dG-dC).poly(dG-dC) and poly (dG-br5dC).poly(dG-br5dC), in presence of ethidlum bromide binds equally well to N-hydroxy-2-aminofluorene. It is suggested that the very low binding of this carcinogen to Z-DNA as compared to B-DNA is due to differences in the dynamic structures of these two forms of DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.