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Nucleic Acids Research, 1983, Vol. 11, No. 15 5257-5266
© 1983

MOLECULAR BIOLOGY

Complete nucleotide sequence of the Escherichia coli gdhA gene

Michael J. McPherson and John C. Wootton

Department of Genetics, University of Leeds Leeds LS2 9JT, UK

Received June 7, 1983. Revised July 11, 1983. Accepted July 11, 1983.

The DNA sequence of the gdhA gene of Escherichia coli K12, which encodes the 447 amino acid polypeptide subunit of NADP-speciflc glutamate dehydrogenase, is presented. The deduced protein sequence is strongly homologous to the corresponding enzyme of the eukaryotic fungus Neurospora crasBa. The upstream DNA sequence includes several overlapping promoter consensus sequences. The downstream DNA sequence contains inverted repeats, predicted as forming long stable stem-loop structures in RNA, homologous to those found in several enterobacterial intergenic regions.


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