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Nucleic Acids Research, 1983, Vol. 11, No. 16 5347-5360
© 1983

MOLECULAR BIOLOGY

The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences

Lambertus P. Woudt, Albert Pastink*, Annemarie E. Kempers-Veenstra, Antonius E.M. Jansen, Willern H. Mager and Rudi J. Planta

Biochemisch Laboratorium, Vrije Universiteit de Boelelaan 1083, 1081 HV Amsterdam, The Netherlands

*Present address: Laboratorium voor Fysiologische Chemie, Rijksuniversiteit Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

Received July 4, 1983. Accepted July 25, 1983.

Sequences coding for histone H3 and H4 of Neurospora crassa could be identified in genomic digests with the use of the corresponding genes from sea urchin and X. laevis as hybridization probes. A 2.6 kb HindIII-generated N. crassa DNA fragment, showing homology with the heterologous histone H3-gene probes was cloned in a charon 21A vector. Using DNA from this clone as a homologous hybridization probe a 6.9 kb Sail-generated DNA fragment was isolated which in addition to the histone H3-gene also contains the gene coding for hietone H4. Several lines of evidence demonstrate the presence of only a single histone H3- as well as a single histone H4-gene in N. crassa. The two genes are physically linked on the genome. DNA sequencing of the N. craasa histone H3- and H4-genes confirmed their identity and, in addition, revealed the presence of one short intron (67 bp) within the coding sequence of the H3-gene and even two introns (68 and 69 bp) within the U4-gene. The amino acid sequences of the N. crassa histories H3 and H4, as deduced from the DNA sequences, and those of the corresponding yeast histones differ only at a few positions. Much larger sequence differences, however, are observed at the DNA level, reflecting a diverging codon usage in the two lower eukaryotes.


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