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Nucleic Acids Research, 1983, Vol. 11, No. 16 5443-5450
© 1983

MOLECULAR BIOLOGY

Multiple 3' ends of the chicken pro {alpha}2(1) collagen gene

Sirpa Aho, Valerie Tate and Helga Boedtker

Department of Biochemistry and Molecular Biology, Harvard University 7 Divinity Avenue, Cambridge, MA 02138, USA

Received May 25, 1983. Accepted July 25, 1983.

The precise location of the 3' ends of the chicken pro {alpha}2(I) collagen gene have been identified by SI nuclease protection of overlapping genomic fragments by calvaria poly A containing RNA and size determination of the protected fragments on DNA sequencing gels. The gene ends 300 and 306 bp and 754 and 777 bp from the translation stop codon. The two sets of ends explain the major and minor pro {alpha}2(I) collagen mSKAs previously observed, which may result from either RNA polymerase readthrough of the first termination site and/or different processing sites.


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