Skip Navigation

This Article
Right arrow Print PDF (2049K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Purvis, I.J.
Right arrow Articles by Moseley, B.E.B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Purvis, I.J.
Right arrow Articles by Moseley, B.E.B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 16 5467-5474
© 1983

ENZYMOLOGY

Isotation and characterisation of Dral, a type II restriction endonuclease recognisting a aequence containing only A:T basepairs, and inhibition of its activity by uv irradiation of substrate DNA

I.J. Purvis and B.E.B. Moseley

Department of Microbiology, University of Edinburgh, School of Agriculture Edinburgh EH9 3JG, UK

Received May 11, 1983. Accepted July 29, 1983.

A type II restriction endonucleasc, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence

5'-T-T-T-{downarrow}A-A-A-3'

3'-A-A-A-{uparrow}T-T-T-5'

and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II restriction endonuclease that recognises a sequence composed solely of A:T basepairs, the isoschizomer Ahalll (1). Ultraviolet irradiation of the DNA substrate at relatively low doses inhibits the activity of Dral by "protecting" the recognition sequence and this may be exploited to give control of partial digestion of DNA by DraI.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.