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Nucleic Acids Research, 1983, Vol. 11, No. 16 5661-5669
© 1983

MOLECULAR BIOLOGY

Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli

Hans Weber and Charles Weissmann

Institut f{euro}r Molekularbiologie I, Universität Zo rich 8093 Zurich, Switzerland

Received July 4, 1983. Accepted July 19, 1983.

We describe a method for the formation of hybrid genes by in vivo recombination between two genes with partial sequence homology. DNA structures consisting of plasmid vector sequences, flanked by the {alpha}2 interferon gene on the one side and a portion of the al interferon gene (homology about 80%) on the other, were transfected into E.coli SK1592. Appropriate resistance markers allowed the isolation of colonies containing circular plasmids which arose by in vivo recombination between the partly homologous interferon gene sequences. Eleven different recombinant genes were identified, six of which encoded new hybrid inter-ferons not easily accessible by recombinant DNA techniques.


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