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Nucleic Acids Research, 1983, Vol. 11, No. 16 5775-5791
© 1983

MOLECULAR BIOLOGY

Maintenance and incompatibility of ptasmids carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication between oriC and asnA

Antoine Raymond Stuitje and Michiel Meijer

Department of Electron Microscopy and Molecular Cytology, University of Amsterdam Plantage Muidergracht 14, 1018 TV Amsterdam, The Netherlands

Received March 30, 1983. Revised July 4, 1983. Accepted July 25, 1983.

Plasmids that replicate only by means of the cloned Escherichia coli replication origin (orlC) are called mlnichromosomes or oriC-plasalds. In this paper It Is shown that sequences located between oriC and asnA are Involved In maintenance and Incompatibility of mlnlchromosomes. These sequences Include part of the 16kD and 17kD genes, previously allocated within this region (1.2).

Transcription towards oriC that Is Initiated at the 16kD promoter, specifically enhances the stability and copy-number of mlnlchromosomes.

Three regions are Involved in mlnichromosome incompatibility. One region, incA, includes the minimal oriC sequence. A second, lncB, maps within a 210 base pairs fragment that overlaps the 16kD promoter. The third, incC, encompasses the 17kD gene. Neither one of the regions expresses Incompatibility on its own, but the additional presence of one of the others is required.

The data presented indicate that sequences of the 16kD and 17kD genes are part of the replication control system of oriC-plasmlds.


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