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Nucleic Acids Research, 1983, Vol. 11, No. 18 6319-6330
© 1983


MOLECULAR BIOLOGY

Nucleotide sequence of IS26, a new prokaryotic mobile genetic etement

Beat Mollet, Shigeru Iida, John Shepherd and Werner Arber

Department of Microbiology, Biozentrum, University of Basel Klingelbergstrasse 70, CH-4056 Basel, Switzerland

Received July 14, 1983. Accepted August 15, 1983.

The DNA sequence of a new IS element, the IS26, is 820 bp long and carries 14 bp perfect terminal inverted repeats. Upon integration, IS26 generates an 8 bp duplication of its target sequence. A large open reading frame within IS26 could code for a protein of 234 amino acids. On its reverse strand, IS26 also carries one large open reading frame, 591 bp long, which contains no stop codon within IS26.


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