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Nucleic Acids Research, 1983, Vol. 11, No. 2 237-250
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence of the amylase gene from Bacillus subtilis§

Maria Yang+, Alessandro Galizzi* and Dennis Henner+

+Department of Molecular Biology, Genentech Inc 460 Point San Bruno Boulevard, South San Fransisco, CA 94080, USA *Instituto di Genetica, Via S. Epifanio 14, 27100 Pavia, Italy

Received November 1, 1982. Revised December 8, 1982. Accepted December 8, 1982.

The gene Coding for amylase (EC.3.2.1.1) has been isolated and sequenced from Bacillus Subtilis by cloning in lambda Charon4A and pBR322. The entire coding Sequence and large preceding and following regions, comprising the presumed transcriptional and translational regulatory regions, were sequenced. The coding sequence shows a large open reading frame with a translated molecular weight of 72,800 and presumed signal sequence of approximately thirty-two amino acids. When the intact gene is present in Escherichia coli, it confers the ability to degrade starch, indicating that the gene is expressed in a functional state.

§Genentech Contribution No. 146


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