Nucleic Acids Research, 1983, Vol. 11, No. 2 369-385
© 1983
MOLECULAR BIOLOGY |
Strcuture and transcription of the nopaline synthase gene region of T-DNA
Department of Biology, Washington University Saint Louis, MO 63110, USA 1Department of Biological Chemistry, Washington University School of Medicin Saint Louis, MO 63110, USA
To whom reprint requests should be sent
Received October 12, 1982. Revised December 10, 1982. Accepted December 10, 1982.
We present the DNA sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pTi T37 DNA from the virulent Agrobacterium tumefaciens strain T37. This region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. The strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.
*Present address: Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 21Q, UK
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Pantaleo, G. Szittya, and J. Burgyan Molecular Bases of Viral RNA Targeting by Viral Small Interfering RNA-Programmed RISC J. Virol., April 15, 2007; 81(8): 3797 - 3806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Miklos, M. F. Alibhai, S. A. Bledig, D. C. Connor-Ward, A.-G. Gao, B. A. Holmes, K. H. Kolacz, V. T. Kabuye, T. C. MacRae, M. S. Paradise, et al. Characterization of Soybean Exhibiting High Expression of a Synthetic Bacillus thuringiensis cry1A Transgene That Confers a High Degree of Resistance to Lepidopteran Pests Crop Sci., January 22, 2007; 47(1): 148 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Vaughn, T. Cavato, G. Brar, T. Coombe, T. DeGooyer, S. Ford, M. Groth, A. Howe, S. Johnson, K. Kolacz, et al. A Method of Controlling Corn Rootworm Feeding Using a Bacillus thuringiensis Protein Expressed in Transgenic Maize Crop Sci., March 28, 2005; 45(3): 931 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-D. M. Chilton and Q. Que Targeted Integration of T-DNA into the Tobacco Genome at Double-Stranded Breaks: New Insights on the Mechanism of T-DNA Integration Plant Physiology, November 1, 2003; 133(3): 956 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Corbin, R. J. Grebenok, T. E. Ohnmeiss, J. T. Greenplate, and J. P. Purcell Expression and Chloroplast Targeting of Cholesterol Oxidase in Transgenic Tobacco Plants Plant Physiology, July 1, 2001; 126(3): 1116 - 1128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-D. Chilton Agrobacterium. A Memoir Plant Physiology, January 1, 2001; 125(1): 9 - 14. [Full Text]
|
|
|
|
 |
|
 Y. Tao, F. Yuan, R. T. Leister, F. M. Ausubel, and F. Katagiri Mutational Analysis of the Arabidopsis Nucleotide Binding Site-Leucine-Rich Repeat Resistance Gene RPS2 PLANT CELL, December 1, 2000; 12(12): 2541 - 2554. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Zhu, D. J. Peterson, L. Tagliani, G. St. Clair, C. L. Baszczynski, and B. Bowen Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides PNAS, July 20, 1999; 96(15): 8768 - 8773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hondred, J. M. Walker, D. E. Mathews, and R. D. Vierstra Use of Ubiquitin Fusions to Augment Protein Expression in Transgenic Plants Plant Physiology, February 1, 1999; 119(2): 713 - 724. [Abstract] [Full Text]
|
|
|
|
|
|
R. Flachmann and W. Kuhlbrandt Crystallization and identification of an assembly defect of recombinant antenna complexes produced in transgenic tobacco plants PNAS, December 10, 1996; 93(25): 14966 - 14971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Donkersloot and J. Thompson Cloning, Expression, Sequence Analysis, and Site-directed Mutagenesis of the Tn5306-encoded N[IMAGE]-(Carboxyethyl)ornithine Synthase from Lactococcus lactis K1 J. Biol. Chem., May 19, 1995; 270(20): 12226 - 12234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Ludwig, B. Bowen, L. Beach, and S. R. Wessler A Regulatory Gene as a Novel Visible Marker for Maize Transformation Science, January 26, 1990; 247(4941): 449 - 450. [Abstract] [PDF]
|
|
|
|
 |
|
 J Callis, M Fromm, and V Walbot Introns increase gene expression in cultured maize cells. Genes & Dev., December 1, 1987; 1(10): 1183 - 1200. [Abstract] [PDF]
|
|
|
|
|
|
D. W. OW, J. R. DE WET, D. R. HELINSKI, S. H. HOWELL, K. V. WOOD, and M. DELUCA Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants Science, November 14, 1986; 234(4778): 856 - 859. [Abstract] [PDF]
|
|
|
|
|
|
A. Caplan, L. Herrera-Estrella, D. Inze, E. Van Haute, M. Van Montagu, J. Schell, and P. Zambryski Introduction of Genetic Material into Plant Cells Science, November 18, 1983; 222(4625): 815 - 821. [Abstract] [PDF]
|
|
|
|
|
|
I. E.J.A. Francois, M. F.C. De Bolle, G. Dwyer, I. J.W.M. Goderis, P. F.J. Woutors, P. D. Verhaert, P. Proost, W. M.M. Schaaper, B. P.A. Cammue, and W. F. Broekaert Transgenic Expression in Arabidopsis of a Polyprotein Construct Leading to Production of Two Different Antimicrobial Proteins Plant Physiology, April 1, 2002; 128(4): 1346 - 1358. [Abstract] [Full Text] [PDF]
|
|