Skip Navigation

This Article
Right arrow Print PDF (430K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Woodcock, D. M.
Right arrow Articles by Cooper, I. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woodcock, D. M.
Right arrow Articles by Cooper, I. A
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 2 489-499
© 1983

MOLECULAR BIOLOGY

Effect of several inhibitors of enzymatic DNA methylation on the in vivo methylation of different classes of DNA sequences in a cultured human cell line

D. M. Woodcock, J. K. Adams, R. G. Allan and I. A Cooper

Haematology Research Unit, Cancer Institute 481 Little Lonsdale Street, Melbourne, 3000 Victoria, Australia

Received October 28, 1982. Revised December 13, 1982. Accepted December 13, 1982.

We have previously demonstrated that , while most enzymatic formation of 5-methycytosine in the DNA of mammalian cells occurs very shortly after strand synthesis, there is also a minor fraction of methylation which occurs in some DNA sequences up to at least several hours after strand synthesis. Using a human cell line , we have examined the effects on these two classes of enzymatic DNA methylation of several compounds which have been reported to be inhibitors of methylation reactions. We have found that cycloleucine, ethionine, and 5'-deoxy-5'-methylthioadenosine (MTA) are a11 effective as inhibitors of enzymatic DNA methylation, but that there is no differentiate effect between the delayed and non-delayed methylation reactions . Tubericidin (7-deaza-adenosine) plus homocysteine inhibited delayed DNA methylation much more than non-delayed methylation (by up to 4 times). By contrast, 5-azacytidine produced a higher level of inhibition of DNA methylation at sites in the DNA in which the methylation occurred very shortly after strand synthesis. Also 5-azacytidine was by far the most potent inhibitor of DNA methylation of the compounds tested. S-Adenosyl-homocysteine and caffeine were found to have no effect on DNA methylation. These results are discussed in relation to the number and specificity of DNA methylases in these cells and to the cellular functions of those DNA sequences in which methylation is delayed for some hours after strand synthesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Rzewuski, K. A. Cornell, L. Rooney, K. Burstenbinder, M. Wirtz, R. Hell, and M. Sauter
OsMTN encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (Oryza sativa L.)
J. Exp. Bot., April 1, 2007; 58(6): 1505 - 1514.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Musri, H. Corominola, R. Casamitjana, R. Gomis, and M. Parrizas
Histone H3 Lysine 4 Dimethylation Signals the Transcriptional Competence of the Adiponectin Promoter in Preadipocytes
J. Biol. Chem., June 23, 2006; 281(25): 17180 - 17188.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.