Skip Navigation

This Article
Right arrow Print PDF (3082K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by James Shen, C.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by James Shen, C.-K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 22 7899-7910
© 1983

MOLECULAR BIOLOGY

Superhetieity induces hypersensitivity of a human polypyrimldine polypurine DNA sequence in the human {alpha}2-{alpha}1 globin intergenic region to SI nudease digestion - high resolution mapping of the clustered cleavage sites

Che-Kun James Shen

Department of Genetics, University of California Davis, CA 95616, USA

Received August 8, 1983. Revised October 17, 1983. Accepted October 17, 1983.

Supercoiled recombinant DNAs containing the human adult a-globin gene region have been probed with nuclease SI in vitro. While agarose gel electrophoresis showed only one predominant, double-stranded cleavage generated by SI within 6 kb of human DNA and 4 kb of pBR322 sequence, a high resolution gel analysis reveals that the unique Sl-hypersensitive locus in the human adult a-globin gene region actually contains more than 15 authenti SI cleavage sites closely spaced together. The mapping approach used here locates the specific SI cleavage sites on both DNA strands at the nucleotide sequence level. Interestingly, most of these sites are mapped within a 90 bp stretch of GC-rich (66%) polypyrimidine'polypurine DNA that is located 1060 to 1150 bp upstream from the al-globin gene. These results provide the first high resolution map of double-stranded Sl-cleavage sites induced within a specific DNA sequence under supercoil strain. The distribution and relative cutting frequencies of these sites mapped are consistent with a slippage mechanism in which the simple repeating sequences are organized into base-mismatched duplex on supercoiled DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.