Skip Navigation

This Article
Right arrow Print PDF (415K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nilsson, B.
Right arrow Articles by Philipson, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nilsson, B.
Right arrow Articles by Philipson, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 22 8019-8030
© 1983

MOLECULAR BIOLOGY

An improved positive selection plasmid vector constructed by ollgonucleotide mediated mutagenesis

Björn Nilsson1, Mathias Uhlén1, Staffan Josephson2, Sten Gatenbeck1 and Lennart Philipson3

1Department of Biochemistry, Royal Institute of Technology S-100 44 2Kabi-Gen Ltd S-112 87 Stockholm, Sweden 3European Molecular Biology Laboratory Postfach 10.2209, 6900 Heidelberg, FRG

Received September 22, 1983. Revised October 25, 1983. Accepted October 25, 1983.

An Escherichia coli plasmid vector, pUN121, has been constructed which allows for positive selection of transformants harboring DNA inserts. The vector is based on plasmid pTR262 (Roberts et al, Gene, 12, (1980), 123–127) in which the tetracycline resistance gene is under transcriptional control of the repressor protein coded by the phage lambda cI gene. This plasmid has been rearranged, using in vitro recombinant techniques including oligonucleotide mediated mutagenesis to yield a smaller plasmid (4.4 kb) with unique cloning sites for EcoRI, XmaI and SmaI in addition to the unique HindHI and Bell sites. The plasmid has a functional ampicillin resistance gene and the new restriction sites (EcoRI, XmaI and Smal) when used for cloning, give rise to tetracycline resistant transformants.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
L. R. Flodgaard, P. Dalgaard, J. B. Andersen, K. F. Nielsen, M. Givskov, and L. Gram
Nonbioluminescent Strains of Photobacterium phosphoreum Produce the Cell-to-Cell Communication Signal N-(3-Hydroxyoctanoyl)homoserine Lactone
Appl. Envir. Microbiol., April 1, 2005; 71(4): 2113 - 2120.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Bouhouche, D. Zickler, R. Debuchy, and S. Arnaise
Altering a Gene Involved in Nuclear Distribution Increases the Repeat-Induced Point Mutation Process in the Fungus Podospora anserina
Genetics, May 1, 2004; 167(1): 151 - 159.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. Kacem, C. De Sousa-D'Auria, M. Tropis, M. Chami, P. Gounon, G. Leblon, C. Houssin, and M. Daffe
Importance of mycoloyltransferases on the physiology of Corynebacterium glutamicum
Microbiology, January 1, 2004; 150(1): 73 - 84.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Wostrikoff, Y. Choquet, F.-A. Wollman, and J. Girard-Bascou
TCA1, a Single Nuclear-Encoded Translational Activator Specific for petA mRNA in Chlamydomonas reinhardtii Chloroplast
Genetics, September 1, 2001; 159(1): 119 - 132.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Merkamm and A. Guyonvarch
Cloning of the sodA Gene from Corynebacterium melassecola and Role of Superoxide Dismutase in Cellular Viability
J. Bacteriol., February 15, 2001; 183(4): 1284 - 1295.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
P. Gourdon, M.-F. Baucher, N. D. Lindley, and A. Guyonvarch
Cloning of the Malic Enzyme Gene from Corynebacterium glutamicum and Role of the Enzyme in Lactate Metabolism
Appl. Envir. Microbiol., July 1, 2000; 66(7): 2981 - 2987.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
M. Yamasaki, K. Miyashita, J. Cullum, and H. Kinashi
A Complex Insertion Sequence Cluster at a Point of Interaction between the Linear Plasmid SCP1 and the Linear Chromosome of Streptomyces coelicolor A3(2)
J. Bacteriol., June 1, 2000; 182(11): 3104 - 3110.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Bourot, O. Sire, A. Trautwetter, T. Touze, L. F. Wu, C. Blanco, and T. Bernard
Glycine Betaine-assisted Protein Folding in a lysA Mutant of Escherichia coli
J. Biol. Chem., January 14, 2000; 275(2): 1050 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
F. C. Boogerd, L. Boe, O. Michelsen, and P. R. Jensen
atp Mutants of Escherichia coli Fail To Grow on Succinate Due to a Transport Deficiency
J. Bacteriol., November 15, 1998; 180(22): 5855 - 5859.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. Paz-Elizur, R. Skaliter, S. Blumenstein, and Z. Livneh
beta*, a UV-inducible Smaller Form of the beta Subunit Sliding Clamp of DNA Polymerase III of Escherichia coli
J. Biol. Chem., February 2, 1996; 271(5): 2482 - 2490.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. K. Larsen and K. Kristiansen
Transcription in Vitro of Tetrahymena Class II and Class III Genes
J. Biol. Chem., March 31, 1995; 270(13): 7601 - 7608.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.