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Nucleic Acids Research, 1983, Vol. 11, No. 23 8197-8203
© 1983

MOLECULAR BIOLOGY

Molecular cloning of the human gastrin gene

Kikuya Kato, Yoshihide Hayashizaki, Yoosuke Takahashi, Seiichi Himeno* and Kenichi Matsubara

Institute for Molecular and Cellular Biology, Osaka University Kita-ku, Osaka 530, Japan *Second Department of Internal Medicine, Medical School, Osaka University Kita-ku, Osaka 530, Japan

Received October 7, 1983. Accepted November 4, 1983.

A genomic clone that contains the human gastrin gene was isolated from a human gene library. Restriction endonuclease mapping and DNA sequencing analysis revealed that this gene is about 0.7 kb long, and has an intron. The intron is located at a position that separates the coding region into the peptlde region essential for biological activities of gastrin and the non-essential, N-terminal peptide region.


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