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Nucleic Acids Research, 1983, Vol. 11, No. 23 8253-8268
© 1983

MOLECULAR BIOLOGY

DNA polymerase and simian virus 40 infection of resting monkey cells: induction of aphidicolin resistant {alpha}-polymerase

Kazushi Tanabe*, Essam Karawya, Joseph W. Fewell, Edward L. Kuff and Samuel H. Wilson

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health Bethesda, MD 20205, USA

Received September 12, 1983. Accepted October 31, 1983.

Tightly confluent monkey cell lines BSC-1 and CV-1 held in stale medium for several days exhibited an extremely low level of thymidine incorporation into cellular DNA. Yet, these cells contained a level of {alpha}-polyraerase equal to about 15% of the level in rapidly dividing cells, and they still were capable of supporting replication of SV40 DNA. SV40 infection and culture in stale medium resulted in a four-fold induction of {alpha}-polymerase in CV-1 cells, whereas no change in {alpha}-polymerase level was observed in BSC-1 cells. Characterization of {alpha}-polymerase partially purified from Infected CV-1 cells revealed that 80–90% of the enzyme activity was aphidicolin resistant. SV40 DNA replication in resting CV-1 cells, however, was aphidicolin sensitive. SV40 infection of resting CV-1 cells may induce an aphidicolin-reslstant enzyme or lead to a modified {alpha}-polymerase species.

*Present address: Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan


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