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Nucleic Acids Research, 1983, Vol. 11, No. 24 8583-8594
© 1983

MOLECULAR BIOLOGY

U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei

Alain Krol1,*, Jean-Pierre Ebel, Jutta Rinke and Reinhard Luhrmann

Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS 15 rue Descartes, 67084 Strasbourg Cedex, France Max-Planck-Institut für Molekulare Genetik Ihnestrasse 63-73, 1000 Berlin 33 (Dahlem), FRG

*To whom correspondence should be addressed

Received October 13, 1983. Revised November 18, 1983. Accepted November 18, 1983.

U1, U2 and U5 RNAs were isolated from pea nuclei with antibody specific for 2,2,7-trimethylguanosine. The nucleotide sequence of the 3'-terminal halves of pea U1 and U2 snRNAs and the complete sequence of five out of the six U5 RNA variants isolated is given. The high number of U5 variants suggest they are encoded by a multigene family containing at least six different genes. Similar secondary structures could be derived for all of the pea U5 RNAs and although the degree of sequence conservation between plant and vertebrate U5 RNAs is as low as 35%, nearly identical secondary structures can be proposed for both RNA groups. All the snRNA species U1, U2 and U5 from pea share a structural domain, the so-called domain A, which is also common to all animal snRNA U1, U2,U4 and U5.Furthermore, a block of 22 consecutive nucleotides is conserved among pea and vertebrate U5 RNAs, from which 11 nucleotides constitute a hairpin-loop with a high number of posttranscriptional modifications. We propose that conservation of this hairpin-loop, together with domain A, is of prime importance for the functioning of U5 RNAs in plant and animal cells.

1Present address: Department of Physiological Chemistry, 587 Medical Sciences Building, 1300 University Avenue, Madison, WI 53706, USA


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