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Nucleic Acids Research, 1983, Vol. 11, No. 24 8719-8734
© 1983

MOLECULAR BIOLOGY

Characterisation of three cDNA clones encoding different mRNAs for the precursor to the small subunit of wheat ribulosebisphosphate carboxylase

Steven M. Smith*,1, John Bedbrook*,2 and Jim Speirs+,3

*Division of Plant Industry, CSIRO P.O. Box 1600, Canberra City, ACT 2601, Australia +Plant Physiology Unit, Division of Food Research, CSIRO, Macquarie University North Ryde, NSW 2113, Australia

3To whom correspondence should be addressed

Received October 24, 1983. Accepted November 17, 1983.

We have isolated and sequenced three cDNA clones for the nuclear-encoded precursor to the small subunit of the chloroplast enzyme, ribulose-1, 5-bisphosphate carboxylase of wheat. The nucleotide sequences of these clones are different, indicating that they are probably derived from three different mRNAs. This finding is consistent with the proposal that this polypeptide is encoded by a multigene family in wheat, in support of similar data reported by Broglie et al. (Bio/Technology 1:55–61, 1983). We deduce that the mature small subunit polypeptide is comprised of 128 amino acids and that its precursor contains an N-terminal transit peptide sequence. The sequences of both the mature small subunit and its transit peptide differ at several positions from those determined by Broglie et al. (1983) from a different wheat cultivar. Different wheat cultivars might therefore contain different small subunit polypeptides. A comparison of nucleotide and amino acid sequences of the small subunit from wheat, pea, soybean and spinach shows that these sequences are not highly conserved, particularly between monocotyledon and dicotyledon species.

1Present address: Botany Department, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK

2Present address: Advanced Genetic Sciences Inc., P.O. Box 3226, Berkeley, CA 94703, USA


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