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Nucleic Acids Research, 1983, Vol. 11, No. 24 8861-8876
© 1983

CHEMISTRY

Interaction of diamidino-2-phenylindole (DAPI) with natural and synthetic nucleic acids

G. Manzini, M.L. Barcellona1, M. Avitabile1 and F. Quadrifoglio*

Istituto di Chimica, Laboratorio di Chimica delle Macromolecole, Università di Trieste 34127 Trieste, Italy 1Istituto di Chimica Biologica, Università di Catania Catania, Italy

*To whom reprint requests should be addressed

Received September 2, 1983. Revised November 9, 1983. Accepted November 23, 1983.

The interaction of DAPI with natural and synthetic polydeoxynucleotides of different base content and sequences was studied with circular dichroism, ultracentrifugation, viscosity and calorimetry. All the polymers show two types of binding. The strength of the interaction and its resistence to ionic strength are related to the content of AT clusters in the chain. On the other hand, sedimentation measurements rule out an intercalation mechanism. A model of DAPI interaction with DNA, similar to that displayed by distamycin and netropsin, is proposed.


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