Skip Navigation

This Article
Right arrow Print PDF (3626K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (86)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Chebath, J.
Right arrow Articles by Revel, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chebath, J.
Right arrow Articles by Revel, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 5 1213-1226
© 1983

MOLECULAR BIOLOGY

Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA

Judith Chebath*, Gilles Merlin, Richard Metz, Phillippe Benech and Michel Revel

Department of Virology, The Weizmann Insitute of Science Rehovot, Israel

Received January 31, 1983. Accepted February 15, 1983.

Treatment of responsive cells by interferons (IFNs) induces within a few hours a rise in the concentration of several proteins and mRNAs. In order to characterize these IFN-induced mRNA species, we have cloned in E. coli the cDNA made from a 17–18S poly(A)+ RNA of human fibroblastoid cells (SV80) treated with IFN-ß. We describe here a pBR322 recombinant plasmid (C56) which contains a 400 bp cDNA insert corresponding to a 18S mRNA species newly induced by IFN. The C56 mRNA codes for a 56,000 dalton protein easily detectable by hybridization-translation experiments. The sequence of 66 of the carboxy-terminal amino-acids of the protein can be deduced from the cDNA sequence. IFNs-{alpha}, ß or {gamma} are able to activate the expression of this gene in human fibroblasts as well as lymphoblastoid cells. The mRNA is not detectable without IFN; it reaches maximum levels (0.1% of the total poly(A)+ RNA) within 4–8 hrs and decreases after 16 hrs.

*Present address: Unite 38 INSERM 27 Bd J. Moulin, 13385, Marseille, Cedex 4, France.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
S. Kim, Y. Choi, F. W. Bazer, and T. E. Spencer
Identification of Genes in the Ovine Endometrium Regulated by Interferon {tau} Independent of Signal Transducer and Activator of Transcription 1
Endocrinology, December 1, 2003; 144(12): 5203 - 5214.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. B. Smith, T. T. Nguyen, H. J. Hughes, H. R. Herschman, D. P. Widney, K. C. Bui, and L. E. Rovai
Glucocorticoid-attenuated response genes induced in the lung during endotoxemia
Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L636 - L647.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
C. E. Samuel
Antiviral Actions of Interferons
Clin. Microbiol. Rev., October 1, 2001; 14(4): 778 - 809.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Patzwahl, V. Meier, G. Ramadori, and S. Mihm
Enhanced Expression of Interferon-Regulated Genes in the Liver of Patients with Chronic Hepatitis C Virus Infection: Detection by Suppression-Subtractive Hybridization
J. Virol., February 1, 2001; 75(3): 1332 - 1338.
[Abstract] [Full Text]

Home page
 J. Gen. Virol.Home page
M. J. Nicholl, L. H. Robinson, and C. M. Preston
Activation of cellular interferon-responsive genes after infection of human cells with herpes simplex virus type 1
J. Gen. Virol., September 1, 2000; 81(9): 2215 - 2218.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
J. Guo and G. C. Sen
Characterization of the Interaction between the Interferon-Induced Protein P56 and the Int6 Protein Encoded by a Locus of Insertion of the Mouse Mammary Tumor Virus
J. Virol., February 15, 2000; 74(4): 1892 - 1899.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.