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Nucleic Acids Research, 1983, Vol. 11, No. 5 1325-1335
© 1983

CHEMISTRY

Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2' -O-tetrahydrofuranyl-5' -O-dimethoxytritylnucleosides

Eiko Ohtsuka, Faculty of Pharmaceutical Sciences, Akio Yamane, Faculty of Pharmaceutical Sciences and Mono Ikehara, Faculty of Pharmaceutical Sciences

Osaka University, 1–6, Yamadaoka Suita, Osaka 565, Japan

Received January 4, 1983. Accepted February 2, 1983.

2'-O-Tetrahydrofurany1-5'-O-dimethoxytrityl-N-protected nucleosides were phosphorylated to give the 3'-(o-chlorophenyl) phosphates which were then condensed with 3',5'-unprotected nucleosides to elongate the chain in the 3'-direction. The 5'-dimethoxytrityl group of these oligonucleotides was selectively deblocked by treatment with zinc bromide. The rate of removal of the dimethoxytrityl group differed in each nucleotide. A dodecamer containing a termination codon UAG, U(AGU)3,AG, was synthesized by elongating the chain in the 5'-direction using the selective dedimethoxytritylation followed by condensation of protected oligonucleotide blocks.


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