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Nucleic Acids Research, 1983, Vol. 11, No. 5 1617-1632
© 1983

ENZYMOLOGY

Synthesis of 2'(3')-O-DL-alanyl hexainosinic acid using T4 RNA ligase: suppression of the enzymic reverse transfer reaction by alkaline phosphatase

Albert T. Profy, Kin-Ming Lo and D.A. Usher

Department of Chemistry, Cornell University Ithaca, NY 14853, USA

Received October 18, 1982. Revised February 1, 1983. Accepted February 1, 1983.

2' (3')-Q-DL-Alanyl (Ip)5I was synthesized by a new method. An alanine ortho ester of inosine 5'-phosphate was added to (Ip)4I using the ATP-independent reaction of T4 RNA ligase, and the product was converted smoothly to the desired ester. The enzymic reverse transfer reaction was conveniently suppressed by the dephosphorylation of the adenosine 5'-phosphate coproduct, catalyzed in situ by alkaline phosphatase.


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