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Nucleic Acids Research, 1983, Vol. 11, No. 6 1919-1930
© 1983

CHEMISTRY

Chain flexibility and hydrodynamics of the B and Z forms of pory(dG-dC).pory(dG-dC)

T.J. Thomas and Victor A. Bloomfield

Department of Biochemistry, University of Minnesota, 1479 Gortner Avenue St. Paul, MN 55108, USA

Received December 14, 1982. Accepted February 24, 1983.

The solution properties of the B and Z forms of poly (dG-dC) .poly (dG-dC) have been measured by static and dynamic laser light scattering. The radius of gyration, persistence length, translational and segnental diffusion coefficients, and the Rouse-Zimm parameters have been evaluated. The persistence length of the Z form determined at 3 M NaCl is about 200 nn compared to 84 and 61 nm respectively for the B forms of poly(d3–dC) .poly-(dS–dC), and calf thymus DNA, both determined at 0.1 M NaCl. The data on persistence length, diffusion coefficients and the Rouse-Ziram parameters indicate a large increase in the chain stiffness of Z DNA compared to the B form. These results are opposite to the ionic strength effects on random sequence native DNAs, for which the flexibility increases with ionic strength and levels off at about 1 M NaCl.


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