Nucleic Acids Research, 1983, Vol. 11, No. 6 1943-1954
© 1983
MOLECULAR BIOLOGY |
Nucleotide sequence of The yeast SUC2 gene for invertase
Department of Human Genetics and Development, College of Physicians and Surgeons of Columbia University 701 West 168th Street, New York, NY 10032, USA.
Received October 18, 1982. Accepted January 3, 1983.
The yeastSUC2 gene is a structural gene for both the secreted and intracellular forms of invertase. We have determined the nucleotide sequence of the coding region and the 5’ and 3’ flanking regions. The coding regions for the signal peptide-containing precursor to secreted invertase and for the intracellular invertase begin at different initiation codons within theSUC2 qene but share the same reading frame. The amino acid sequences predicted for the two forms of invertase from the nucleotide sequence are consistent with the properties of the purified enzymes. Potential sites for glycosylation of the secreted invertase are identified.
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