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Nucleic Acids Research, 1983, Vol. 11, No. 7 2111-2120
© 1983

MOLECULAR BIOLOGY

DNA sequence of the excision sites of a mitochondrial plasmid from senescent Podospora anserina

Donald J. Cummings and Richard M. Wright

Department of Microbiology and Immunology, University of Colorado Health Sciences Center Dencer, CO 80262, USA

Received December 6, 1982. Revised February 28, 1983. Accepted February 28, 1983.

During senescence in Podospora anserina, specific gene regions of the mitochondrial genome are excised and amplified. The most prevalent, termed {alpha}–event senDNA, is a 2600 bp circular molecule which is excised from the contiguous Hae III fragments 23,14 region of the mitochondrial DNA restriction map. We have cloned {alpha}–DNA plasmid from races s+ and A+ as well as the genomic fragments Hae III 23,14 and have sequenced those regions which constitute the {alpha}–junction sites. We have found that one excision site (J1) is located 24 bp from the proximal Hae III 23 restriction site and the other (J2) 172 bp from the distal Hae III 14 site. Flanking the {alpha}–DNA sequences on the mitochondrial genome, there are 10 bp palindromic sequences: CAATATATTG, ending 3 bases from the Jl site, and ATTATATAAT which starts 8 bases from the J2 site. Neither of these 10 bp palindromes are present on the {alpha}–DNA plasmid. Abutting the Jl site on the {alpha}–DNA there is a 5 bp sequence (GTGCT) which 1s repeated 8 bp downstream. In joining the two distal Jl and J2 sites, a 7 bp repeat (ACGTGCG) is produced. These results are discussed within the context of site–specific recombination.


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