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Nucleic Acids Research, 1984, Vol. 12, No. 11 4481-4492
© 1984

MOLECULAR BIOLOGY

Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence

Marion R. Fung, Rob M. Campbell and Ross T.A. MacGillivray

Department of Biochemistry, University of British Columbia Vancouver, BC V6T 1W5, Canada

Received March 7, 1984. Revised May 11, 1984. Accepted May 11, 1984.

Thirty thousand colonies of a bovine liver cDNA library were screened with a mixture of synthetic oligodeoxyribonucleotides coding for bovine factor X. Five positive colonies were identified, and plasmid DNA was isolated. Cleavage with restriction endonucleasea showed that these plasmids (designated pBX1-5) contained inserts of 1530bp, 770bp, 700bp, 1100bp and 930bp. DNA sequence analysis of the plasmid with the largest insert (pBX1) confirmed that bovine factor X cDNAs had been cloned. The cDNA sequence predicts that factor X is synthesized as a single chain precursor in which the light and heavy chains of plasma factor X are linked by the dipeptide Arg-Arg. The cDNA sequence also predicts that factor X is synthesized with a prepro leader peptide. We propose that at least five specific proteolytic events occur during the conversion of prepro-factor X to plasma factor Xa.


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