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Nucleic Acids Research, 1984, Vol. 12, No. 12 4977-4985
© 1984

MOLECULAR BIOLOGY

The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene

John Swindle*, Janet Ajioka*, Dean Dawson+, Richard Myers, Dana Carroll and Costa Georgopoulos

Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, University of Utah Salt Lake City, UT 84132, USA

Received March 12, 1984. Accepted April 18, 1984.

The nusB (groNB) gene product of Escherichia coli plays a pivotal role in allowing bacteriophage {lambda} N protein to function aa an antiterminator of mRNA transcription and in modulating host gene expression. In addition it is essential for bacterial viability since mutations in it result in a cold-sensitivity phenotype for growth. We have previously cloned the nusB gene and shown it to code for a 14,500-Hr protein. Here we present the primary DNA sequence of the nusB gene. From the sequence we deduce that it codes for a slightly basic protein (21 basic as opposed to 20 acidic amino acids) composed of 139 amino acids with a cumulative 15,689-Mr. The predicted N-terminal amino acid sequence as well as the overall amino acid composition agrees well with that of the purified protein.

*Present address: Unite d'Immunoparasitologie, Institut Pasteur, 75724 Paris, Cedex 15, France

+Present address: Department of Biological Chemistry, Harvard Medical School, Boston, Ma 02115, USA.


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