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Nucleic Acids Research, 1984, Vol. 12, No. 12 5037-5047
© 1984

MOLECULAR BIOLOGY

Nucieotide sequence of a 2 kbp BamH I fragment of Vicia faba chloroplast DNA containing the genes for threonine, glutamic add and tyrosine transfer RNAs

M. Kuntz*, J.H. Weil and A. Steinmetz+

Institut de Biologie Moleculaire et CeUulaire du CNRS, Université Louis Pasteur 15 rue René Descartes, F-67084 Strasbourg, France

+To whome correspondence should be addressed

Received April 26, 1984. Accepted May 23, 1984.

The entire nucleotide sequence of a 2014 bp BamH I fragment from broad bean ( Vicia faba ) chloroplast DNA containing the genes for tRNAThr (trnT), tRNAGlu (trnE) and tRNATyr (trnY) has been determined. The tRNAGlu and tRNATyr genes are separated by only 60 bp and are probably part of the same transcriptional unit. The tRNAThr gene is located on the complementary strand, 876 bp away from the tRNAGlu gene. This fragment also contains an open reading frame of 82 codons, as well as a series of AT-rich, direct and inverted repeats.

*Present address: Max-Planck-institut für Züchtungsforschung, Abteilung Schell, D-5000 Köln (Vogelsang) 30 (FRG)


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