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Nucleic Acids Research, 1984, Vol. 12, No. 13 5165-5173
© 1984

MOLECULAR BIOLOGY

Alteration of apparent restriction endonuclease recognition specificities by DNA methylases

Michael Nelson, Chantal Christ and Ira Schildkraut

New England Biolabs, Inc. 32 Tozer Road, Beverly, MA 01915, USA

Received June 5, 1984. Accepted June 12, 1984.

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I in Adeno-2 DNA and for Acc I and Hinc II in pBR322 DNA by specifically methylating all but one recognition sequence.


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