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Nucleic Acids Research, 1984, Vol. 12, No. 15 6231-6246
© 1984

MOLECULAR BIOLOGY

Viroid replication: equilibrium association constant and comparative activity measurements for the viroid-polymerase interaction

Thomas C. Goodman*, Luitgard Nagel, Winfried Rappold**, Günther Klotz+ and Detlev Riesner#

Institut für Physikalische Biologie, Universitt Düsseldorf D-4000 Düsseldorf +Abteilung für Virologie, Universitt Ulm D-7900 Ulm, FRG

#TO whom reprint requests should be addressed

Received June 12, 1984. Accepted July 16, 1984.

The binding and replication of purified potato spindle tuber viroid (PSTV) by DNA-dependent RNA polymerase II from wheat germ was studied in analytical ultracentrifugation experiments and in vitro transcription assays. The equilibrium association constant for the viroid-polymerase interaction is 1.9 x 107 M–1. Both ultraviolet and fluorescent monitoring during the sedimentation experiments showed two distinguishable viroid-polymerase complexes. These are interpreted as resulting from a 1:1 and 2:1 enzyme-to-viroid binding stoichiometry. A265/A280 ratios across the sedimenting boundaries, the sedimentation velocity of the complexes, as well as electron microscopic data support this interpretation. The role of viroid secondary structure in enzyme binding and polymerization is discussed in the light of these results and compared with binding and polymerization data for virusoid RNA, single- and double-stranded RNA, and double-stranded DNA.

*Present address: CIBA-GEIGY Corp., P.O. Box 12257, Research Triangle Park, NC 27709-2257, USA.

**The work presented here is in partial fulfillment of the requirements for the Ph.D. degree.


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