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Nucleic Acids Research, 1984, Vol. 12, No. 16 6603-6614
© 1984

MOLECULAR BIOLOGY

Poly(pyrimidine) poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH

Jeremy S. Lee, Mary L. Woodsworth, Laura J.P. Latimer and A.Richard Morgan1

Department of Biochemistry, University of Saskatchewan Saskatoon, Saskatchewan, S7N 0W0 1Department of Biochemistry, University of Alberta Edmonton, Alberta, T6G 2H7, Canada

Received April 19, 1984. Revised July 20, 1984. Accepted July 20, 1984.

Poly(pyrimidine), poly(purine) tracts have been discovered in the 5-flanking regions of many eucaryotic genes. They may be involved in the regulation of expression since they can be mapped to the nuclease-sensitive sites of active chromatin. We have found that poly(pyrimIdlne).poly(purine) DNA8 which contain 5-methylcytosine (e.g. poly[d(Tm5C)]·poly[d(GA)]) will form a triplex at a pH below 8. In contrast, the unmethylated analogue, poly[d(TC)]·poly[(GA)] only forms a triplex at pits below 6. Synthetic containing repeating trinucleotides and poly[d(Um5C)]·poly[d(GA)] behave in a similar manner. Thus the stability of a triplex can be controlled by methylation of cytosine. This suggests a model for the regulation of expression based upon specific triplex formation on the 5'-side of eucaryotic genes.


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