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Nucleic Acids Research, 1984, Vol. 12, No. 17 6709-6726
© 1984

MOLECULAR BIOLOGY

Attachment of repeated sequences to the nuclear cage

D.A. Jackson, P.R. Cook and S.B. Patel

Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford, OX1 3RE, UK

Received June 18, 1984. Revised August 4, 1984. Accepted August 21, 1984.

Nuclear DNA is probably organized into loops by attachment to a sub-structure in vivo. When HeLa cells are lysed in Triton and 2M NaCl the resulting nucleoids contain naked DNA which is supercoiled so the loops must remain intact. We have attempted to identify sequences responsible for attaching these loops to the nuclear sub-structure by progressively detaching DNA with various nucleases. Fragments at the 5' end of the ribosomal RNA locus, and a variety of transcribed and repeated sequences, are shown to lie relatively close to attachment points. This implies that sequences cannot be arranged randomly. However no "attachment sequence" could be identified.


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