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Nucleic Acids Research, 1984, Vol. 12, No. 18 6995-7006
© 1984

MOLECULAR BIOLOGY

Immunoglobulin genes of the x light chain type from two human lymphoid cell lines are closely related

H.-Gustav Klobeck, Gabriele Combriato and Hans G. Zachau

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universitat München, FRG

Received July 4, 1984. Revised August 28, 1984. Accepted August 28, 1984.

As a first step in our studies of functionally rearranged K genes of man we cloned the germline JK-CK region from placenta DNA employing a mouse JK clone as hybridization probe. Subclones of the human JK-CK region were then used to characterize and clone the rearranged K genes of the lymphoid cell lines Walker and Daudi. The Walker cell line contains one rearranged and one germline K allele (K+,KO; ref. 1). Only one K gene was found in Daudi cells (K+). Restriction mapping and DNA sequencing showed, that the rearranged K genes from both cell lines are closely related. These features make the two cell lines particularly suitable for studies on the chromatin structure of K light chain genes. The 5' flanks of the two genes (388 bp) are identical while there is a 12 % divergence between the VK gene segments themselves. This situation may reflect somatic mutation processes and/or gene conversion like events.


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