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Nucleic Acids Research, 1984, Vol. 12, No. 18 7007-7021
© 1984

MOLECULAR BIOLOGY

Correlation between DNase I hypersensitive sites and putative regulatory sequences in human immunoglobulin genes of the x light chain type

Valerij A. Pospelov, H.-Gustav Klobeck and Hans G. Zachau

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, FRG

Received July 4, 1984. Revised August 28, 1984. Accepted August 28, 1984.

The human lymphoid cell lines Walker and Daudi constitute a particularly suitable system for studies on the chromatin structure of K light chain genes (see preceding paper). The rearranged and non-rearranged alleles of Walker cells were found to be about equally sensitive towards digestion with DNAase I. A DNAase I hypersensitive site was mapped 0.13 kb upstream of the leader segment of the rearranged VK genes; it comprises a region in which promoter-like regulatory elements were discovered recently. Additional hypersensitive sites are located further upstream. A hypersensitive site in the JK-CK intron coincides with a putative tissue specific enhancer element. A hypersensitive region downstream of CK overlaps with the cleavage/polyadenylation recognition signal which is flanked by sequences related to the above mentioned putative regulatory sequences. The coincidence between DNAase I hypersensitive sites and those sequences may be functionally significant.


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