Skip Navigation

This Article
Right arrow Print PDF (1559K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hensgens, L. A.M.
Right arrow Articles by Benne, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hensgens, L. A.M.
Right arrow Articles by Benne, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 19 7327-7344
© 1984

MOLECULAR BIOLOGY

The sequence of the gene for cytochrome c oxidase subunit I, a frameshift containing gene for cytochrome c oxidase subunit II and seven unassigned reading frames in Trypanosoma brucei mitochondrial maxi-cirle DNA

Lambert A.M. Hensgens+, Just Brakenhoff, Berend F.De Vries, Paul Sloof, Marijke C. Tromp*, Jacques H. Van Boom* and Rob Benne§

Section for Gene Structure and Expression, Laboratory of Biochemistry, University of Amsterdam P.O. Box 60.000, 1005 GA Amsterdam *Organic Chemistry Laboratory, Gorlaeus Laboratories P.O.Box 9502. 2300 RA Leiden. The Netherlands

§To whom correspondence should be addressed

Received July 30, 1984. Accepted September 13, 1984.

A 9.2 kb segment of the maxi-circle of Trypanosoma brucei mito-chondrial DNA contains the genes for cytochrome c oxidase subunits I and II (coxI and coxII) and seven Unassigned Reading Frames ("URFs").

The genes for coxI and coxII display considerable homology at the aminoacid level (38 and 25%, respectively) to the corresponding genes in fungal and mammalian mtDNA, the only striking point of divergence being an unusually high cysteine content (about 4.5%). The reading frame coding for cytochrome c oxidase subunit II is discontinuous: the C-terminal portion of about 40 amlnoacids, is present in the DNA-sequence in a -1 reading frame with respect to the N-terminal moiety.

URF5, 8 and 10, show a low but distinct homology (about 20%) to mammalian mitochondrial URF-1, 4 and 5, respectively. In URF5, the first AUG is found at codon 145, whereas extensive homology to mammalian URF-1 sequences occurs upstream of this position. The possibility exists that UUG can serve as an initiator codon.

URF7 and URF9 have a highly unusual aminoacid composition and do not possess AUG or UUG initiator codons. These URFs probably do not have a protein-coding function.

The segment does not contain conventional tRNA genes.

+Present address: Istituto di Anatomia Comparata Biologia Generale, Genetica, Via romana 17, Firenze, Italy.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. A. Keightley, B. H. Zimmermann, M. W. Mather, P. Springer, A. Pastuszyn, D. M. Lawrence, and J. A. Fee
Molecular Genetic and Protein Chemical Characterization of the Cytochrome ba(3) from Thermus thermophilus HB8
J. Biol. Chem., September 1, 1995; 270(35): 20345 - 20358.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T Jacks and H. Varmus
Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting
Science, December 13, 1985; 230(4731): 1237 - 1242.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.