Skip Navigation

This Article
Right arrow Print PDF (2372K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Verbeet, M. Ph.
Right arrow Articles by Planta, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verbeet, M. Ph.
Right arrow Articles by Planta, R. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 2 1137-1148
© 1984

MOLECULAR BIOLOGY

A conserved sequence element is present around the transcription initiation site for RNA polymerase A in Saccharomycetoideae

Martin Ph. Verbeet, Jacobus Klootwijk, Harm van Heerikhuizen, Ruud D. Fontijn, Erno Vreugdenhil and Rudi J. Planta

Biochemisch Laboratorium, Vrije Universiteit, de Boelelaan 1083 1081 HV Amsterdam, The Netherlands

Received September 22, 1983. Revised December 6, 1983. Accepted December 6, 1983.

To identify DNA elements involved in the initiation of rRNA transcription in yeast we located the start site of the rRNA operon of Kluyveromyces lactis and Hansenula wingei both members of the Saccharomycetoideae by S1 nuclease analysis and determined the surrounding nucleotide sequences.

Comparison of these sequences with those of Saccharomyces carlsbergensis S. cerevisiae and S. rosei (all belonging to the same yeast subfamily) reveals an identical sequence at the site of transcription initiation from position +1 to +7 which is part of a larger conserved region extending from position –9 to +23; the conserved heptanucleotide sequence is supposed to constitute an important part of the promoter for yeast RNA polymerase A. The non-transcribed spacers (NTS) upstream of position –9 have diverged strongly with the exception of two short elements around positions –75 and –135. The external transcribed spacer (ETS) downstream of position +23 is largely conserved between K. lactis, S. rosei and S. carlsbergensis except for a divergent region around position +75. On the other hand, the ETS of H. wingei has diverged significantly.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.