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Nucleic Acids Research, 1984, Vol. 12, No. 2 887-899
© 1984

ENZYMOLOGY

The nucleotide sequence recognized by the Escherichia coli A restriction and modification enzyme

Manfred Kröger and Gerd Hobom

Institut fur Biologie III der Universität Freiburg Schanzlestrasse 1, D-7800 Freiburg, FRG

Received November 14, 1983. Accepted December 6, 1983.

The nucleotide recognition sequence for the restriction-modification enzyme of Escherichia coli A (EcoA) has been determined to be GAG-7N-GTCA. This sequence is fairly similar, but distinctly different from the two other type I restriction enzyme recognition sites known for E.coii B and E.coii K12, respectively. N6-adenosine methylation has been observed at nucleotide positions 2 and 12 within that sequence after modification by EcoA. As a reference point for mapping the single EcoA site in lambda, the position of lambda point mutation Oam29 has been determined also.


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