Skip Navigation

This Article
Right arrow Print PDF (1197K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (64)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Unger, B.
Right arrow Articles by Wells, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Unger, B.
Right arrow Articles by Wells, R. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 20 7693-7703
© 1984

Articles

Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes

Bernhard Unger, Gerd Klock, Wolfgang Hillen and Robert D. Wells

Institut für Physikalische Biologic, Universität Düsseldorf, Universitätsstrasse I, D-4000 Düsseldorf, FRG

Received July 24, 1984. Accepted October 3, 1984.

The tetracycline resistance determinant of RA1 was cloned. It consists of at least two genes oriented with opposite polarity, tetA for resistance and tetR for regulation. The transcriptional control sequence was identified and analyzed. It consists of overlapping promotors with divergent orientation and a tandem arrangement of operators. Nucleotide sequencing revealed two open reading frames. One codes for a protein which was identified as a Tet repressor by comparing its primary structure with those of other let repressors. The RA1 tetR gene codes for 218 amino acids with a calculated molecular weight of 24.4kDa. In the primary sequence of the RA1-, p5C101-, Tn10-, and RP1/Tn1721- encoded let repressors, 36% of the amino acids are identical. This homology is clustered within the first 150 amino acids, 49% of which are identical among all four proteins. These results are discussed with respect to their structure and function In comparison to other DNA binding proteins.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
J. L. Ramos, M. Martinez-Bueno, A. J. Molina-Henares, W. Teran, K. Watanabe, X. Zhang, M. T. Gallegos, R. Brennan, and R. Tobes
The TetR Family of Transcriptional Repressors
Microbiol. Mol. Biol. Rev., June 1, 2005; 69(2): 326 - 356.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. Parke and L. N. Ornston
Hydroxycinnamate (hca) Catabolic Genes from Acinetobacter sp. Strain ADP1 Are Repressed by HcaR and Are Induced by Hydroxycinnamoyl-Coenzyme A Thioesters
Appl. Envir. Microbiol., September 1, 2003; 69(9): 5398 - 5409.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Dalsgaard, A. Forslund, D. Sandvang, L. Arntzen, and K. Keddy
Vibrio cholerae O1 outbreak isolates in Mozambique and South Africa in 1998 are multiple-drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons
J. Antimicrob. Chemother., December 1, 2001; 48(6): 827 - 838.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
L. GUARDABASSI, L. DIJKSHOORN, J.-M. COLLARD, J.E. OLSEN, and A. DALSGAARD
Distribution and in-vitro transfer of tetracycline resistance determinants in clinical and aquatic Acinetobacter strains
J. Med. Microbiol., October 1, 2000; 49(10): 929 - 936.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W Hinrichs, C Kisker, M Duvel, A Muller, K Tovar, W Hillen, and W Saenger
Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance
Science, April 15, 1994; 264(5157): 418 - 420.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.