Skip Navigation

This Article
Right arrow Print PDF (2603K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cantor, C. R.
Right arrow Articles by Efstratiadis, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cantor, C. R.
Right arrow Articles by Efstratiadis, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 21 8059-8072
© 1984

MOLECULAR BIOLOGY

Possible structures of homopurine-homopyrimidine S1-hypersensitive sites

Charles R. Cantor and Argiris Efstratiadis

Department of Human Genetics and Development, Columbia University 701 West 168th Street, New York, NY 10032, USA

Received August 8, 1984. Revised October 4, 1984. Accepted October 4, 1984.

S1 nuclease hypersensitive sites in the 5' flanking regions of eukaryotic genes, present in small artificial supercoiled DNA circles, reside in homo-purine-homopyrimidine stretches. The hierarchical behavior which these sites exhibit is consistent with the notion that they act as sinks of torsional free energy. By employing DMS as a single-strand-specific reagent, we show that these sites (despite their S1 sensitivity) are regions of duplex DNA. A simple thermodynamic treatment indicates that the high torsional stress in the small DNA circles is almost certain to be relieved by the formation of alternate DNA structures. The same treatment places some constraints on the types and sizes of the regions with alternate conformation. While no definitive structural conclusions can be drawn, left-handed helices seem most consistent with the extent and the pattern of sensitivity to S1 nuclease.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
V. S. Susulic, L. LaVallette, E. Duzic, L. Chen, D. Shuey, S. K. Karathanasis, and K. E. Steiner
Expression of the Human {beta}3-Adrenergic Receptor Gene in SK-N-MC Cells Is Under the Control of a Distal Enhancer
Endocrinology, May 1, 2001; 142(5): 1935 - 1949.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Charron, J.-P. Julien, and V. Bibor-Hardy
Neuron Specificity of the Neurofilament Light Promoter in Transgenic Mice Requires the Presence of DNA Unwinding Elements
J. Biol. Chem., October 27, 1995; 270(43): 25739 - 25745.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
H Htun and J. Dahlberg
Topology and formation of triple-stranded H-DNA
Science, March 24, 1989; 243(4898): 1571 - 1576.
[Abstract] [PDF]

Home page
 ScienceHome page
H Htun and J. Dahlberg
Single strands, triple strands, and kinks in H-DNA
Science, September 30, 1988; 241(4874): 1791 - 1796.
[Abstract] [PDF]

Home page
 ScienceHome page
B. Johnston
The S1-sensitive form of d(C-T)n.d(A-G)n: chemical evidence for a three-stranded structure in plasmids
Science, September 30, 1988; 241(4874): 1800 - 1804.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.