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Nucleic Acids Research, 1984, Vol. 12, No. 21 8115-8128
© 1984

MOLECULAR BIOLOGY

Hormonal regulation of transcription of rDNA: use of nucleoside thiotriphosphates to measure initiation in isolated nuclei

Zhen-yong Zhang, E.Aubrey Thompson and Michael R. Stallcup

Department of Biology, University of South Carolina Columbia, SC 29208, USA

Received July 10, 1984. Revised August 29, 1984. Accepted September 27, 1984.

Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (ß-S)triphosphates. Initiation of pre-rRNA was completely resistant to {alpha}-amanitin but was inhibited by either actinomycin D or heparin. Experiments with P1798 mouse lymphoma cells indicated that the antiproliferative effects of glucocorticoids on lymphoid cells includes a reduction in the ability of nuclei to initiate pre-rRNA chains.


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