Skip Navigation

This Article
Right arrow Print PDF (5322K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Harrison, B.
Right arrow Articles by Zimmerman, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harrison, B.
Right arrow Articles by Zimmerman, S. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1984, Vol. 12, No. 21 8235-8251
© 1984

ENZYMOLOGY

Polymer-stimulated ligation: enhanced ligation of oligo- and polynucleotides by T4 RNA ligase in polymer solutions

Barbara Harrison and Steven B. Zimmerman

Laboratory of Molecular Biology, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, National Institutes of Health Bethesda, MD 20205, USA

Received August 13, 1984. Accepted October 5, 1984.

The effects of macromolecular crowding were tested on several reactions catalyzed by T4 RNA ligase. The rate of cyclization of oligoriboadenylates was stimulated up to 10-fold by relatively high concentrations of several polymers (polyethylene glycol (PEG) 8000 or 20,000; bovine plasma albumin; Ficoll 70). In addition, higher concentrations of PEG 8000 or PEG 20,000 allowed the novel formation of large linear products from the oligoriboadenylates. Also stimulated by high concentrations of PEG 8000 were the rate at which T4 RNA ligase joined p(dT)10 to oligoriboadenylates and the rate at which the enzyme activated p(dT)n by transfer of an adenylyl moiety from ATP to the oligonucleo-tides. These results with T4 RNA ligase are compared to earlier studies on the effects of crowding on DNA ligases.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
S. A. KAZAKOV, S. V. BALATSKAYA, and B. H. JOHNSTON
Ligation of the hairpin ribozyme in cis induced by freezing and dehydration.
RNA, March 1, 2006; 12(3): 446 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. P. So, R. F. B. Turner, and C. A. Haynes
Increasing the efficiency of SAGE adaptor ligation bydirected ligation chemistry
Nucleic Acids Res., July 6, 2004; 32(12): e96 - e96.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. DAS, I. HARVEY, L. L. CHU, M. SINHA, and J. PELLETIER
Full-length cDNAs: more than just reaching the ends
Physiol Genomics, July 17, 2001; 6(2): 57 - 80.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.