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Nucleic Acids Research, 1984, Vol. 12, No. 22 8579-8593
© 1984

MOLECULAR BIOLOGY

A retroviral provirus closely associated with the Ren-2 gene of DBA/2 mice

David W. Burt, Alistair D. Reith and William J. Brammar

ICI/University Joint Laboratory, Department of Biochemistry, University of Leicester Leicester LE1 7RH, UK

Received September 6, 1984. Revised October 19, 1984. Accepted October 19, 1984.

We have determined the entire nucleotide sequence of an intra-cisternal A particle (IAP) genome, associated with the Ren-2 gene of DBA/2 mice. This genome (MIARN) displays features common to other IAP retroviral-like genomes. Long terminal repeats (LTRs) are approximately 430 base pairs (bp) in length and show typical retroviral U3-R-U5 organisation, though the R-region, at 120 bp, is much larger than the average IAP. This difference probably arose by the amplification of a pyrimidine-rich sequence, by a slippage-mispairing mechanism. Flanking the 5' LTR is a sequence complementary to a phenylalanine tRNA, strongly conserved in all rodent IAP genomes and probably required to prime the initiation of (–) strand synthesis. Flanking the 3' LTR, is a purine-rich sequence probably required for (+) strand synthesis. The tRNA binding site (TBS) is flanked by six tandem copies of a sequence homologous to the TBS. The relationship of the MIARN element to other IAP genomes and the significance of its association with the highly expressed Ren-2 is discussed.


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