Nucleic Acids Research, 1984, Vol. 12, No. 22 8711-8721
© 1984
MOLECULAR BIOLOGY |
Binary Agrobacterium vectors for plant transformation
Plant Breeding Institute Maris Lane, Trumpington, Cambridge CB2 2LQ, UK
Received July 24, 1984. Revised October 25, 1984. Accepted October 25, 1984.
A vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. It utilizes the trans acting functions of the vir region of a co-resident Ti plasmid in Agrobacterium tumefaciens to transfer sequences bordered by left and right T-DNA border sequences into the nuclear genome of plants. The T-region contains a dominant selectable marker gene that confers high levels of resistance to kanamycin, and a lac alpha-complementing region from M13mpl9 that contains several unique restriction sites for the positive selection of inserted DNA.
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