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Nucleic Acids Research, 1984, Vol. 12, No. 24 9299-9307
© 1984

MOLECULAR BIOLOGY

Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II

Hiroshi Gushima2, Shuhay Yasuda3, Eiichi Soeda3, Masami Yokota2, Masatoshi Kondo2 and Akira Kimura1

1Research Institute for Food Science, Kyoto University Uji, Kyoto 611, Japan 2Central Research Laboratory of Yamanouchi Pharmaceutical Company 1-1-8 Azusawa, Itabashi-ku, Tokyo 174, Japan 3National Institute of Genetics 1,111 Yata, Mishima 411, Japan

Received October 24, 1984. Accepted November 7, 1984.

The nucleotide sequence of the cloned DNA, 1,478 bp in length coding for glutathione synthetase (GSH-II) of E.coli B has been determined. Amino acid and nucleotide sequence analyses have assigned the open reading frame for GSH-II, starting with the ATG near its 5' terminus. The molecular weight caluculated from the predicted amino acid sequence is 35,559 daltons, being in good agreement with that of a GSH-II subunit estimated by the SDS-PAGE method. Several signal sequences conserved in the promoter regions of E.coli were found in the non-coding regions of the gsh-II gene. They include the Shine-Dalgarno sequence, the Pribnow box and the sequence conserved in the "–35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequences were present, followed by 6 successive T's. These structural features found in the non-coding regions have suggested to be involved in regulatory functions for the gsh-II gene expression.


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