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Nucleic Acids Research, 1984, Vol. 12, No. 3 1489-1500
© 1984

MOLECULAR BIOLOGY

Site-specific nicking within the adenovirus inverted terminal repetition

Kuan-Chih Chow and George D. Pearson

Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331, USA

Received October 3, 1983. Revised January 9, 1984. Accepted January 9, 1984.

Site-specific nicking occurs on the l-Strand, but not on the r-strand, of the adenovirus left inverted terminal repeat. Nicks are presumably introduced into double- or single-Stranded DNA by a cellular endonuclease in an ATP independent reaction. The consensus nick site has the sequence:

GGRGYG GGRNRNGTG.


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