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Nucleic Acids Research, 1984, Vol. 12, No. 3 1543-1558
© 1984

MOLECULAR BIOLOGY

In monkey COS cells only the TATA box and the cap site region are required for faithful and efficient initiation of the fibroin gene transcription

K. Tokunaga*, S. Hirose and Y. Suzuki{dagger}

Laboratoiy of Cell Differentiation, National Institute for Basic Biology Myodaiji, Okazaki City 444, Japan

{dagger}To whom correspondence should be addressed

Received December 12, 1983. Revised January 9, 1984. Accepted January 9, 1984.

The DNA sequences necessary for faithful and efficient initiation of transcription of Bombyx mori fibronin gene have been studied in vivo COS using monkey COS celia and a SV40 origin vector. Transcriptional analysis of 5' deletion genes and of exact substitution genes including a series of single base substitution mutants indicates that the TATA box and the cap region are required for faithful and efficient initiation of the fibroio gene traoscription in vivo, whereas far upatream sequences and the CAAT-like sequencea are not required. In addition, single base substitution genes of the cap site region have clearly shown that there exists a strict sequence requirement of the cap region of the fibroin gene. These results define the basic promoter of the fibroin gene to the region spanning the TATA box and the cap site region.

*Present address: Chiba Cancer Center Research Institute, 666-2, Nitona-cho, Chibs 280, Japan


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