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Nucleic Acids Research, 1984, Vol. 12, No. 3 1609-1619
© 1984

MOLECULAR BIOLOGY

Diadenosine 5' ,5' ''-P1, P4 in devdoplng embryos of Artemia

Alexander G. McLennan* and Mark Prescott

Department of Biochemistry, University of Liverpool P.O. Box 147, Liverpool L69 3BX, UK

*To whom all correspondence should be addressed

Received November 24, 1983. Accepted January 3, 1984.

Diadenosine 5',5''' P1, P4 - tetraphosphate (Ap has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 104 -106 times lower than that of the guanine analogue Gp4 G. The unexpectedly high level of Ap4 A in dormant cysts of 2.37 pmol/106 cells can be reduced to 0.03 pmol/106 cells by decapsulatlon and storage in saturated NaCl. When development is reinitiated, the Ap4 A content of the decapsulated embryos undergoes a rapid 125 -fold increase, reaching a maximum of 3.79 pmol/106 cells at the point of emergence when DNA replication begins. If replication is delayed by hypoxia, the Ap4 A level is adjusted in order to reach the same maximum value when replication finally begins. As replication proceeds, the level of Ap4 A declines again. Unlike mammalian cells, Ap4 A In Artemia is less metabolically labile than ATP. These results are consistent with the suggested role of Ap4 A in the initiation of DNA synthesis.


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