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Nucleic Acids Research, 1984, Vol. 12, No. 3 1627-1640
© 1984

MOLECULAR BIOLOGY

Expression of Ty-lacZ fusions in Saccharomyces cerevisiae

B.A. Bowen1, A.M. Fulton, M.F. Tuite2, S.M. Kingsman and A.J. Kingsman*

Department of Biochemistry South Parks Road, Oxford OX1 3QU, UK

*To whom correspondence should be sent

Received November 8, 1983. Accepted December 19, 1983.

We have determined the nucleotide sequence of about 520 bp spanning the 5' delta regions (Figure 1) of two Tyl elements. There is an open reading frame running out of the deltas for at least 180 nucleotides into the internal region of each element. The functional significance of these open reading frames has been tested by fusing them to a defective E.coli lacZ gene. Expression of B-galactosidase in yeast transformants containing these fusions shows that Tyl elements contain functional translation signals.

1Present address: Department of Applied Genetics, John Innes Institute, Colney Lane, Norwich, UK

2Present address: Biological Laboratory, University of Kent, Canterbury, UK


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